The handsome actor, 53, looked dapper in a classic tuxedo as his stunning wife, 47, commanded attention in a semi-sheer black lace gown.
The appearance came after Oscars host Jimmy Kimmel reignited his hilarious feud with Damon by concluding the broadcast with Messi, the dog from Anatomy of a Fall, urinating on Damon's Hollywood Walk of Fame star.
In this whimsical moment, the canine lifts his leg over the star before strolling away, playfully implying that Kimmel might have orchestrated the comical stunt.
The bit added another chapter to the enduring 'feud' between Damon and the host of Jimmy Kimmel Live!.
This playful rivalry has persisted for years, tracing its origins back to 2005 when Kimmel joked that his show couldn't make room for Damon to be a guest.
In a recent CNN interviewbefore the Oscars, Kimmel teased Damon for skipping this year's award show despite starring in the most-nominated movie, Oppenheimer.
'He is not nominated for an Oscar. He was in Oppenheimer. Everyone else was nominated except for him,' Kimmel said.
He added, 'What really happened? He couldn’t get a ticket. It’s one of the saddest things I’ve ever heard. He asked if he could come and they told him, "Sorry, nominees only."'
Last year, he took a playful swipe at Damon during a comedic exchange with Jessica Chastain.
'Was it difficult filming your movie The Martian because your co-star Matt Damon can't read and smells like dog medicine?' Kimmel hilariously asked.
The handsome actor, 53, looked dapper in a classic tuxedo as his stunning wife, 47, commanded attention in a semi-sheer black lace gown
The appearance came after Oscars host Jimmy Kimmel reignited his hilarious feud with Damon by concluding the broadcast with Messi , the dog from Anatomy of a Fall, urinating on Damon's Hollywood Walk of Fame star
In this whimsical moment, the canine lifts his leg over the star before strolling away, playfully implying that Kimmel might have orchestrated the comical stunt
Jessica quipped, 'I didn't really have a lot of scenes with him, so it was fine.'
In his Sunday opening monologue, he honored Messi, the canine actor portraying Snoop in this year's Best Original Screenplay winner, Anatomy of a Fall.
'Messi, who, even though he’s a dog, may have given the performance of the year in Anatomy of a Fall,' Kimmel joked.
'Messi has an overdose scene, if you’ve seen it you’ve known it. It’s incredible,' Kimmel added.
'Honestly I haven’t seen a French actor eat his own vomit like that since Gérard Depardieu.'
Oscar winners 2024 full list - see who received trophies for this year's Academy Awards
Best Picture
American Fiction
Anatomy of a Fall
Barbie
The Holdovers
Killers of the Flower Moon
Maestro
Oppenheimer - WINNER
Past Lives
Poor Things
The Zone of Interest
Oppenheimer was the biggest winner of the night with seven including top honor Best Picture;Emma Thomas, Christopher Nolan, and Charles Roven are seen
Best Director
Justine Triet, Anatomy of a Fall
Martin Scorsese, Killers of the Flower Moon
Christopher Nolan, Oppenheimer - WINNER
Yorgos Lanthimos, Poor Things
Jonathan Glazer, The Zone of Interest
Actress in a Leading Role
Annette Bening, Nyad
Lily Gladstone, Killers of the Flower Moon
Sandra Huller, Anatomy of a Fall
Carey Mulligan, Maestro
Emma Stone, Poor Things - WINNER
Emma Stone earned a shock win in theActress in a Leading Role category for Poor Things
Actor in a Leading Role
Bradley Cooper, Maestro
Colman Domingo, Rustin
Paul Giamatti, The Holdovers
Cillian Murphy, Oppenheimer - WINNER
Jeffrey Wright, American Fiction
Actress in a Supporting Role
Emily Blunt, Oppenheimer
Danielle Brooks, The Color Purple
America Ferrera, Barbie
Jodie Foster, Nyad
Da’Vine Joy Randolph, The Holdovers - WINER
Da’Vine Joy Randolph triumphed in theActress in a Supporting Role category for The Holdovers
Actor in a Supporting Role
Sterling K. Brown, American Fiction
Robert De Niro, Killers of the Flower Moon
Robert Downey Jr., Oppenheimer - WINNER
Ryan Gosling, Barbie
Mark Ruffalo, Poor Things
Animated Feature Film
The Boy and the Heron - WINNER
Elemental
Nimona
Robot Dreams
Spider-Man: Across the Spider-Verse
Cinematography
El Conde
Killers of the Flower Moon
Maestro
Oppenheimer,Hoyte van Hoytema - WINNER
Poor Things
Original Screenplay
Anatomy of a Fall, Justine Triet and Arthur Harari - WINNER
The Holdovers, David Hemingson
Maestro, Bradley Cooper and Josh Singer
May December, Samy Burch (Story b Samy Burch & Alex Mechanik)
Past Lives, Celine Song
Adapted Screenplay
American Fiction, Cord Jefferson - WINNER
Barbie, Greta Gerwig & Noah Baumbach
Oppenheimer, Christopher Nolan
Poor Things, Tony McNamara
The Zone of Interest, Jonathan Glazer
Cord Jefferson won Adapted Screenplay for American Fiction
Achievement in Production Design
Barbie
Killers of the Flower Moon
Napoleon
Oppenheimer
Poor Things,Production Design: James Price and Shona Heath; Set Decoration: Zsuzsa Mihalek - WINNER
Achievement in Sound
The Creator
Maestro
Mission: Impossible - Dead Reckoning Part One
Oppenheimer
The Zone of Interest,Tarn Willers and Johnnie Burn - WINNER
Original Song
'The Fire Inside' from Flamin' Hot
'I'm Just Ken' from Barbie
'It Never Went Away' from American Symphony
'Wahzhazhe (A Song for My People)' from Killers of the Flower Moon
'What Was I Made For?' from Barbie, Billie Eilish and Finneas O'Connell - WINNERS
Billie Eilish and Finneas O'Connell earned Original Song for Barbie's What Was I Made For?
Original Score
American Fiction
Indiana Jones and the Dial of Destiny
Killers of the Flower Moon
Oppenheimer,Ludwig Göransson - WINNER
Poor Things
Live Action Short Film
The After
Invincible
Knight of Fortune
Red, White and Blue
The Wonderful Story of Henry Sugar,Wes Anderson and Steven Rales - WINNER
Animated Short Film
Letter to a Pig
95 Senses
Our Uniform
Pachyderme
WAR IS OVER! Inspired by the Music of John and Yoko - WINNER
WAR IS OVER! Inspired by the Music of John and Yoko won Animated Short Film;Dave Mullins and Brad Booker pictured
Documentary Feature Film
Bobi Wine: The People's President
The Eternal Memory
Four Daughters
To Kill a Tiger
20 Days in Mariupol - WINNER
Documentary Short Film
The ABCs of Book Banning
The Barber of Little Rock
Island in Between
The Last Repair Shop - WINNER
Nǎi Nai and Wài Pó
International Feature Film
Io Capitano, Italy
Perfect Days, Japan
Society of the Snow, Spain
The Teachers' Lounge, Germany
The Zone of Interest, United Kingdom - WINNER
The Zone Of Interest took home two Oscars includingInternational Feature Film; Jonathan Glazer and James Wilson are pictured
Makeup and Hairstyling
Golda
Maestro
Oppenheimer
Poor Things,Nadia Stacey, Mark Coulier and Josh Weston - WINNER
Society of the Snow
Achievement in Costume Design
Barbie
Killers of the Flower Moon
Napoleon
Oppenheimer
Poor Things, Holly Waddington - WINNER
Achievement in Film Editing
Anatomy of a Fall
The Holdovers
Killers of the Flower Moon
Oppenheimer, Jennifer Lame - WINNER
Poor Things
Visual Effects
The Creator
Godzilla Minus One,Takashi Yamazaki, Kiyoko Shibuya, Masaki Takahashi and Tatsuji Nojima - WINNER
Guardians of the Galaxy Vol. 3
Mission: Impossible - Dead Reckoning Part One
Napoleon
Godzilla Minus One won the Visual Effects award asMasaki Takahashi, Takashi Yamazaki, Kiyoko Shibuya and Tatsuji Nojima are pictured
